Preface Part I Gene Cloning Chapter 1 Introduction to Gene Cloning 1.1 Brief Overview 1.2 Historical Events 1.3 Significance and Alertness Chapter 2 Gene and Genome 2.1 Discovery of Hereditary Factor 2.2 Basic Knowledge of Gene 2.3 Sequence Signals Involved with Gene Expression 2.4 About Genomic and Metagenomic Studies Chapter 3 Basic Techniques for Experiments with DNA 3.1 Enzymatic Tools for Gene Manipulation 3.2 Oligo-nucleotide Tools 3.3 DNA Purification 3.4 Electrophoresis 3.5 In Vitro Synthesis of DNA 3.6 DNA Sequencing 3.7 Macromolecular Hybridization and Blotting 3.8 PCR Techniques Chapter 4 Cloning Procedure 4.1 Basic Procedure of Cloning 4.2 Selection of Cloning Vectors 4.3 Preparation of Donor DNA 4.4 Joining of Donor and Vector by Ligation 4.5 Selection Host Cell 4.6 Introduction of Recombinant DNA into Host Cells 4.7 Selection,Screening and Sub-cloning 4.8 Derivative Technology Based on Cloning Chapter 5 Application and Impacts 5.1 Initiation of Recombinant DNA Industry 5.2 General Aspects of Application 5.3 Contribution and Impact Part II Cell Engineering Chapter 6 Introduction on Cell Engineering 6.1 Brief Overview 6.2 Essentials of Cell Engineering 6.3 Progress Clues Chapter 7 Cell Fusion and Hybridization 7.1 General Aspects 7.2 Protoplast Technique 7.3 Basic Methods of Cell Fusion 7.4 Fusant Screening 7.5 Application of Cell Fusion Chapter 8 Plant Tissue and Cell Culture 8.1 Plant Tissue Culture System 8.2 Gene Transfer to Plants 8.3 Artificial Seeds and Virus-free Plants Chapter 9 A nimal Tissue and Cell Culture 9.1 Establishment of Animal Tissue Culture System 9.2 Gene Transfer to Animal Cell 9.3 Animal Hybrids and Hybridoma 9.4 Approaches of Stem Cell Research Chapter 10 Application Examples from Cell Engineering 10.1 Bioproducts from Cell Engineering 10.2 Breeding of New Agronomic Varieties 10.3 Transgenic Animals as Models for Medical Research Part III Enzyme Technology Chapter 11 Fundamental Nature of Enzyme 11.1 Definition,Basic Nature and Classification of Enzyme 11.2 Determination of Enzyme Activities 11.3 Factors Affecting Enzyme Activity 11.4 Resources of Enzyme 11.5 Uses of Enzyme Chapter 12 Enzyme Biosynt hesi sand Products 12.1 Bio-resources of Enzyme 12.2 About Enzyme Synthesis 12.3 Enzyme Fermentation and Products Chapter 13 Enzyme Recov ery & Purification 13.1 Pretreatment of Crude Enzyme Sample 13.2 Removing of Impurities and Sample Concentration 13.3 Chromatographic Separation 13.4 Polishing of Enzyme 13.5 Rational Formulation of Final Enzyme Products Chapter 14 Enzymatic Conversion 14.1 Enzymatic Conversion 14.2 Immobilization of Enzyme and Cells 14.3 Enzymatic conversion in non-aqueous Phase Chapter 15 New Approaches of Enzyme Technology 15.1 Modification on Enzymes 15.2 Enzyme Carriers for Immobilization 15.3 Enzyme Inhibitors 15.4 Ribozyme(Non-protein Enzymes) Part IV Industrial Fermentation Chapter 16 Principles of Fermentation 16.1 Fermentation Principles 16.2 Fermentation Products and Application 16.3 Benefits from the Fermentation Industry Chapter 17 Industrial Microorganisms 17.1 Types and Characters of Industrial Microorganisms 17.2 Breeding of Industrial Strains 17.3 Industrial Microbial Growth and Culture Media 17.4 Inoculation of Industrial Strains 17.5 Preservation of Industrial Strains Chapter 18 Microbial Metabolism and Fermentation Kinetics 18.1 Metabolism Related to Basic Pathway 18.2 Metabolic Regulation and Control 18.3 Stoichiometry of Fermentation Process 18.4 Fermentation Kinetics Chapter 19 Bioreactors and Auxiliary Equipments 19.1 Structure and Function of Bioreactors 19.2 Auxiliary Equipment 19.3 Probes and Sensors Chapter 20 Fermentation Down-Stream Technology 20.1 General Aspects 20.2 Harvest and Pretreatment 20.3 Isolation and Purification 20.4 Product Polishing 20.5 Waste Treatment and Cleaner Production Chapter 21 Progress in Fermentation Engineering 21.1 Approaches of Up-stream Technology 21.2 Process Optima 21.3 Development of Down-stream Technology Appendix Terms Translation Related Nobel Laureates Further Reading 后记
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