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　　Enzyme Engineering(Third Edition)introduces the basic theories,basic technologies recent developments and growth trends in the fields of enzyme production and applications.This book in 10 Chapters covers the introduction,enzyme production by microbial fermentation,enzyme production by animal and plant cell cultures,extraction,Separation ana purification of enzyme,enzyme molecular modification,immobilization of enzyme,Cells and protoplasts,enzyme Catalysis in non-aqueous phases,enzyme directed evolution,enzyme reactors,and applications of enzymes.A review and thinking issues are given at the end of every chapter. 　　This book can be used as a text book for the students specializing in biotechnology,bioengineering,biochemical engineering,enzyme engineering,fermentation engineering,bioscience etc.It can also be used as reference by the teachers。scientists,engineers and technicians working in the fields related to enzyme engineering. 　　This book has been translated from the third Chinese edition of“Enzyme Engineering” published by Science Press,Beijing,China.

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• Preface
  Chapter 1 Introduction
  1.1 Conception of Enzyme and Its Development
  1.2 The Characteristics of Enzyme Action
  1.3 Factors that Affecting Enzyme Action
  1.4 Classification and Nomenclature of Enzymes
  1.5 Determination of Enzyme Activity
  1.6 Methods for Enzymes Production
  1.7 Development Survey of Enzyme Engineering
  Review and Thinking
  Chapter 2 Enzyme Production by Microbial Fermentation
  2.1 Regulation of Enzyme Biosynthesis in Microbial Cells
  2.2 Characteristic of Microbes for Enzyme Production
  2.3 Fermentation Process Conditions and Its Control
  2.4 Enzyme Fermentation Kinetics
  2.5 Enzyme Production by Immobilized Cell Fermentation
  2.6 Enzyme Production by Immobilized Protoplast Fermentation
  Review and Thinking
  Chapter 3 Enzyme Production by Animal and Plant Cell Cultures
  3.1 The Regulation of Enzyme Biosynthesis in Animal and Plant Cells
  3.2 Enzyme Production by Plant Cell Culture
  3.3 Enzyme Production by Animal Cell Culture
  Review and Thinking
  Chapter 4 Extraction,Separation and Purification of Enzymes
  4.1 Cell Disruption
  4.2 Enzyme Extraction
  4.3 Precipitation Separation
  4.4 Centrifugal Separation
  4.5 Filtration and Membrane Separation
  4.6 Chromatographic Separation
  4.7 Electrophoresis Separation
  4.8 Extraction Separation
  4.9 Crystallization
  4.10 Concentration and Drying
  Review and Thinking
  Chapter 5 Enzyme Molecular Modification
  5.1 Metal Ion Substitution Modification
  5.2 Macromolecular Binding Modification
  5.3 Side Chain Group Modification
  5.4 Peptide Chain Limited Hydrolysis Modification
  5.5 Nucleotide Chain Cleavage Modification
  5.6 Amino Acid Substitution Modification
  5.7 Nucleotide Substitution Modification
  5.8 Physical Modification
  5.9 Application of Enzyme Molecular Modification
  Review and Thinking
  Chapter 6 Immobilization of Enzymes,Cells and Protoplasts
  6.1 Enzyme Immobilization
  6.2 Cell Immobilization
  6.3 Protoplast Immobilization
  Review and Thinking
  Chapter 7 Enzyme Catalysis in Non-aqueous Phases
  7.1 The Main Scopes of Enzyme Catalysis in Non-aqueous Phase
  7.2 Effects of Water and Organic Solvents on Enzyme Catalysis in Organic Media
  7.3 The Catalytic Characters of Enzymes in Organic Media
  7.4 Conditions of Enzyme Catalytic Reaction in Organic Media and Its Control
  7.5 Application of Enzyme Catalysis in Non-aqueous Phase
  Review and Thinking
  Chapter 8 Enzyme Directed Evolution
  8.1 The characteristics of Enzyme Directed Evolution
  8.2 Random Mutation of Enzyme Gene
  8.3 Directed Selection of Enzyme Gene Mutants
  8.4 The Applications of Enzyme Directed Evolution
  Review and Thinking
  Chapter 9 Enzyme Reactors
  9.1 Types of Enzyme Reactors
  9.2 Choice of Enzyme Reactors
  9.3 Design of Enzyme Reactors
  9.4 Enzyme Reactor Operation
  Review and Thinking
  Chapter 10 Applications of Enzymes
  10.1 Enzymes Application in Pharmaceutical Industry
  10.2 Application of Enzymes in Food
  10.3 Enzyme Application in Light and Chemical Industries
  10.4 Enzyme Application in Environmental Protection
  10.5 Enzyme Application in Biotechnology
  Review and Thinking
  Main references