Immunoassay has a prominent position in rapid and sensitive detection. As understanding of the application of immunoassay in food safety increases,and consumer awareness of hazardous factors in food grows,demand for high sensitive immunoassay techniques will rise remarkably. Food Immunochemistry and Immunology reviews the latest developments in immunoassay of typical pollutes in foods.The first part(9 chapters)relate to the primary knowledge of various immunoassay techniques and the preparation of immuno-molecules including antigen and antibody. The second part,in four chapters,considers the common pollutes in food such as agrochemicals,veterinary drugs,mycotoxins and other emerging contaminants and outlines the latest scientific achievements(especially in China). Food Immunochemistry and Immunology will provide professionals and researchers with a useful reference source.
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目录
Preface Chapter 1 Introduction 1.1 History of immunology and immunological techniques 1.1.1 The empirical period of immunology 1.1.2 The period of classical immunology 1.1.3 The period of early modern immunology 1.1.4 The period of modern immunology 1.2 The extensive application of immunological techniques 1.2.1 Immunology and medicine 1.2.2 Immunology and biology 1.2.3 Immunology and the development of biotechnology 1.3 Present situation and prospects of immunology 1.3.1 The in-depth development of immunology at molecule level 1.3.2 The interaction of immune system and neuroendocrine system 1.3.3 The infiltration of immunology into biology,basic medicine,clinical medicine and preventive medicine 1.3.4 The applied research of immunology promoted the development of biotechnology References Chapter 2 The Basic Principles of Antigen-antibody Reaction 2.1 The general principles of antigen-antibody binding 2.1.1 Antigen 2.1.2 Antibody 2.1.3 The general principles of antigen-antibody binding 2.1.4 The characteristics of antigen-antibody reaction 2.2 The factors affecting Ag-Ab binding 2.2.1 Antibody 2.2.2 Antigen 2.2.3 Electrolytes 2.2.4 pH 2.2.5 Temperature 2.3 The main application of Ag-Ab binding reaction 2.3.1 The detection of complement 2.3.2 The detection of immune globulin 2.3.3 The detection of immune complexes 2.3.4 The detection of cytokines References Chapter 3 Fabrication and Purification of Antibodies 3.1 Polyclonal antibodies 3.1.1 Preparation principle 3.1.2 The choice of species 3.1.3 Processing the antigen 3.1.4 Immunization of animals 3.2 Monoclonal antibodies 3.2.1 Principle 3.2.2 The choice of animal and immunization 3.2.3 Cell fusion 3.2.4 Screening of hybridoma cell and detecting antibody 3.2.5 Subcloning of hybridoma cell 3.2.6 Hybridoma cell freezing and thawing 3.2.7 Mass production of monoclonal antibody 3.2.8 Identification of monoclonal antibody 3.3 Purification of antibody 3.3.1 Principle 3.3.2 Purification methods References Chapter 4 Antibody Engineering 4.1 Cell engineering antibody 4.1.1 Animal immunizing 4.1.2 Cell fusion 4.1.3 Screening of hybridoma cell 4.2 Genetic engineering antibody 4.2.1 Chimeric antibody 4.2.2 Humanized antibody 4.2.3 Fully humanized antibody 4.2.4 Small molecule antibodies and antibody fusion protein 4.2.5 Bispecific diabody 4.3 Antibody library technology 4.3.1 Application of antibody library 4.3.2 Phage display human antibody library 4.3.3 The construction of phage display human antibody 4.4 Applications of antibody References Chapter 5 Labelled Immunoassay Technique 5.1 Radioimmunoassay 5.1.1 Technical evolvement 5.1.2 Principle 5.1.3 Basic reagents and technical methods 5.2 Enzyme immune techniques 5.2.1 Summary 5.2.2 Preparation of enzyme-labelled antibody 5.2.3 Amplification of enzyme immunoassay 5.2.4 Application and development trend of enzyme immunoassay 5.3 Fluoroimmunoassay 5.3.1 Brief review 5.3.2 Labelling method 5.4 Chemiluminescence immunoassay 5.4.1 Principle 5.4.2 Classification of CLIA method 5.4.3 Applications and prospects References Chapter 6 Time-Resolved Fluorescence Immunoassay 6.1 Principles 6.1.1 Homogeneous fluorescence analysis 6.1.2 Time-resolved fluorescence and rare earth element 6.2 Applications 6.2.1 Application in immunology 6.2.2 Applications in microbiology 6.2.3 Applications in molecular biology 6.2.4 Applications in hormone detection 6.2.5 Other applications 6.3 Multi-labelled time-resolved fluorescence immunoassay 6.3.1 Principle 6.3.2 Application 6.4 Enzyme-amplified time-resolved fluoroimmunoassay 6.4.1 Principles 6.4.2 Application References Chapter 7 Molecular Immunology and Immunogenetics 7.1 DNA isolation and purification 7.1.1 Introduction 7.1.2 Several common methods for enrichment and purification of DNA 7.2 Nucleic acid probe technology 7.2.1 Overview 7.3 Nucleic acid molecular hybridization 7.3.1 Principles and types 7.3.2 Application 7.4 Construction and screening of cDNA library 7.4.1 cDNA library and construction 7.4.2 cDNA library screening 7.5 HLA gene matching and typing technology 7.5.1 HLA gene matching 7.5.2 Matching methods for HLA-II genes References Chapter 8 Immunoblotting Technique 8.1 Introduction 8.1.1 SDS-PAGE 8.1.2 Transfer 8.1.3 Enzyme immunoassay 8.1.4 Notices of Western blot 8.2 Molecular imprinting technique(MIT) 8.2.1 Overview of molecular imprinting 8.2.2 Methyl testosterone and molecular imprinting technique 8.2.3 The problem and outlook of molecularly imprinting technique 8.3 Applications of immunobloting test References Chapter 9 Immunoelectron Microscopy 9.1 Overview 9.1.1 Tissue fixation and sampling 9.1.2 Immune staining 9.1.3 Embedding 9.2 Ferritin immune technology 9.2.1 Basic principles 9.2.2 Extraction and purification of ferritin 9.2.3 Combination of ferritin and immunoglobulin 9.2.4 Preparation of electron microscope specimens 9.3 Other immunoelectron microscopy 9.3.1 Colloidal gold labelled method for immunoelectron microscopy 9.3.2 Scanning immunoelectron microscopy 9.3.3 Freeze-etching immunoelectron microscopy 9.4 Situ hybridization for electron microscopy 9.4.1 Application of isotope-labelled cRNA probe with situ hybridization electron microscope in chromosome 9.4.2 Application of biotin-labelled DNA probe with situ hybridization electron microscopy 9.4.3 Application of digoxin-labelled rRNA probe in situ hybridization electron microscope References Chapter 10 Pesticide Immunoassay 10.1 Organophosphorus pesticides 10.1.1 Overview 10.1.2 Immunoassay of organophosphorus pesticide 10.2 Immunoassy for organochlorine pesticides 10.2.1 Overview 10.2.2 Analysis method 10.3 Carbamates 10.3.1 Overview 10.3.2 Immunoassay for carbamates 10.4 Pyrethroid insecticides 10.4.1 Overview 10.4.2 Immunoassay for pyrethroids 10.5 Herbicides pesticides 10.5.1 Overview 10.5.2 Immunoassay for herbicides 10.6 Other pesticides 10.6.1 Triazole pesticides 10.6.2 Benzimidazole pesticides 10.6.3 Immunoassay 10.7 Immunoassay based on class specific antibody References Chapter 11 Immunoassay for Veterinary Drugs 11.1 Aminoglycoside antibiotics 11.1.1 Introduction 11.1.2 Immunoassay 11.2 Macrolide antibiotics 11.2.1 Introduction 11.2.2 Immunoassay of MALs 11.3 Chloramphenicols drugs 11.3.1 Introduction 11.3.2 Immunoassay 11.4 β-lactam antibiotics 11.4.1 Introduction 11.4.2 Analytical method 11.5 Nitrofuran drugs 11.5.1 Introduction 11.5.2 Immunoassay for nitrofurans 11.6 Quinolones 11.6.1 Introduction 11.6.2 Immunoassay for QNs 11.7 Sulfonanlides 11.7.1 Introduction 11.7.2 Immunoassay for SAs 11.8 Tetracyclines 11.8.1 Introduction 11.8.2 Immunoassay for TCs 11.9 Anthelmintic(worm)agents 11.9.1 Introduction 11.9.2 Immunoassay for anthelmintics 11.10 Anticoccidial drugs 11.10.1 Introduction 11.10.2 Immunoassay for anticoccidial drugs 11.11 Anabolic hormones 11.11.1 Introduction 11.11.2 Immunoassay for ASs 11.12 β-Agonist 11.12.1 Introduction 11.12.2 Immunoassay for β-agonist 11.13 Other drugs 11.13.1 Benzodiazepines 11.13.2 Retention analysis for polyettler antibiotics 11.13.3 Immunoassay for olaquindox and its metabolites 11.14 Multi-residal immunoassay for veterinary drugs References Chapter 12 Biological Toxin Immunoassay 12.1 Animal toxins 12.1.1 Overview 12.1.2 Immunoassy of biological toxins 12.2 Fungi and mycotoxins 12.2.1 Overview 12.2.2 Analytical methods 12.3 Bacteria and bacterial toxins 12.3.1 Overview 12.3.2 Immunoassay for bacterial toxins 12.4 Plant toxins 12.4.1 Introduction 12.4.2 Immunoassay for plant toxins 12.5 Algae and phycotoxins 12.5.1 Overview 12.5.2 Immunoassay References Chapter 13 Immunoassay for Other Residues 13.1 Dioxins 13.1.1 Introduction 13.1.2 Immunoassay for doxins 13.2 Polybrominated biphenyl 13.2.1 Introduction 13.2.2 Immunoassay for PBDEs 13.3 Sudan red and para red 13.3.1 Introduction 13.3.2 Immunoassay for Sudan red 13.4 Malachite green 13.4.1 Introduction 13.4.2 Immunoassay for MG 13.5 Melamine 13.5.1 Introduction 13.5.2 Immunoassay of MEL 13.6 Phthalate(ester of phthalic acid) 13.6.1 Introduction 13.6.2 Immunoassay for PAE 13.7 Bisphenol A 13.7.1 Introduction 13.7.2 Immunoassay for BPA 13.8 Heavy metal contamination 13.8.1 Introduction 13.8.2 Immunoassay References Colored Figures
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