本书从2003年开始在世界范围内（主要是欧美）被用作课程教材，足以证明其影响力。这是第3版，在以前版本的基础上进行了大幅修改。 本书的核心理念在于：设计生物过程首先要基于对生物系统的模拟，因此，需要引入生物科学里数学、定量的方法，以期和工程科学完善结合，以指导最佳生物过程的设计。相对于国内大多数生物反应工程教材，本书更注重基础、注重数学方法。这是特别值得借鉴的。 在基础科学部分，本书设计第2、3、4、5章。分别从生物分子、物料守恒、生物反应热力学和生化反应系统四方面介绍生物过程的科学实质。 第6、7、8章，结合前面四章内容，系统总结了酶、细胞、细胞群落三个生物反应过程。侧重于用数学方法描述酶催化、细胞（群落）转化过程，特别重视基础科学和工程科学的结合。 第9、10、11章介绍生物反应中的工程问题，主要包括发酵过程的设计、气液传质以及生物过程放大。 本书理念先进、层次清楚，基础和工程结合紧密，特别值得引入国内教育领域。
样章试读
目录
1.1 Note on Nomenclature
2 Chemicals from Metabolic Pathways
2.1 The Biorefinery
2.1.1 Ethan01 Production
2.1.2 Production of Platform Chemicals in the Biorefinery
2.2 The Chemistry of Metabolic Pathways
2.2.1 The Currencies of Gibbs Free Energy and of Reducing Power
2.2.2 Glycolysis
2.2.3 Fermentative Metabolism:Oxidation of NADH in Anaerobic Processes
2.2.4 The TCA Cycle:Provider of Building Blocks and NADH/FADH2
2.2.5 Production of ATP by Oxidative Phosphorylation
2.2.6 The Pentose Phosphate Pathway:A Multipurpose Metabolic Network
2.2.7 Summary of the Primary Metabolism of Glucose
2.3 Examples of Industrial Production of Chemicals by Bioprocesses
2.3.1 Amino Acids
2.3.2 Antibiotics
2.3.3 Secreted Proteins
2.4 Design of Biotech Processes:Criteria for Commercial Success
2.4.1 Strain Design and Selection
2.4.2 Criteria for Design and Optimization of a Fermentation Process
2.4.3 Strain Improvement
2.5 The Prospects of the Biorefinery
Problems
References
3 Elemental and Redox BaIances
3.1 The Continuous,Stirred Tank Reactor
3.1.1 Mass Balances for an Ideal,Steady-State Continuous Tank Reactor
3.2 Yield Coefficients
3.3 B1ack Box Stoichiometries
3.4 Degree of Reduction Balances
3.4.1 Consistency Test of Experimental Data
3.4.2 Redox Balances Used in the Design of Bioremediation Processes
3.5 Systematic Analysis of Black Box Stoichiometries
3.6 Identification of Gross Measurement Errors
Problems
References
4 Thermodynamics of Bioreactions
4.1 Chemical Equilibrium and Thermodvnamic State Functions
4.1.1 Changes in Free Energy and Enthalpy
4.1.2 Free Energy Changes in Bioreactions
4.1.3 Combustion:A Change in Reference State
4.2 Heat of Reaction
4.2.1 Nonequilibrium Thermodynamics
4.2.2 Free Enervy Reclaimed by Oxidation in the Electron Transfer Chain
4.2.3 Production of ATP Mediated by F0-F1 ATP Synthase
Problems
References
5 Biochemical Reaction Networks
5.1 Basic Concepts
5.1.1 Metabolic Network with Diverging Branches
5.1.2 A Formal,Matrix-Based Description of Metabolic Networks
5.2 Growth Energetics
5.2.1 Consumption of ATP for Cellular Maintenance
5.2.2 Energetics of Anaerobic Processes
5.2.3 Energetics of Aerobic Processes
5.3 Flux Analysis in Large Metabolic Networks
5.3.1 Expressing the Rate of Biomass Formation
5.3.2 The Network Structure and the Use of Measurable Rates
5.3.3 The Use of Labeled Substrates
Problems
References
6 Enzyme Kinetics and Metabolic Control Analysis
6.1 Enzyme Kinetics Derived from the Model of Michaelis-Menten
6.2 More Complicated Enzyme Kinetics
6.2.1 Variants of Michaelis-Menten Kinetics
6.2.2 Cooperativity and Allosteric Enzymes
6.3 Biocatalysis in Practice
6.3.1 Laboratory Studies in Preparation for an Industrial Production Process
6.3.2 Immobilized Enzymes and Diffusion Resistance
6.3.3 Choice of Reactor Type
6.4 Metabolic Control Analysis
6.4.1 Definition of Control Coefficients for Linear Pathways
6.4.2 Using Connectivity Theorems to Calculate Control Coefficients
6.4.3 The Influence of Effectors
6.4.4 Approximate Methods for Determination of the CJi
Problems
References
7 Growth Kinetics of Cell Cultures
7.1 Model Structure and Complexity
7.2 A General Structure for Kinetic Models
7.2.1 Specification of Reaction Stoichiometries
7.2.2 Reaction Rates
7.2.3 Dynamic Mass Balances
7.3 Unstructured Growth Kinetics
7.3.1 The Monod Model
7.3.2 Multiple Reaction Models
7.3.3 The Influence of Temperature and pH
7.4 Simple Structured Models
7.4.1 Compartment Models
7.4.2 Cybemetic Models
7.5 Derivation of Expression for Fraction of Repressor-free 0perators
7.6 Morphologically Structured Models
7.6.1 0scillating Yeast Cultures
7.6.2 Growth of Filamentous Microorganisms
7.7 Transport Through the Cell Membrane
7.7.1 Facilitated Transport,Exemplified by Eukaryotic Glucoside Permeases
7.7.2 Active Transport
Problems
References
8 Population Balance Equations
Problems
References
9 Design of Fermentation Processes
9.1 Steady-state Operation of the STR
9.1.1 The Standard CSTR with vf=ve=v
9.1.2 Productivity in the Standard CSTR
9.1.3 Productivity in a Set of Coupled,Standard CSTR's
9.1.4 Biomass Recirculation
9.1.5 Steadv-State CSTR with Substrates Extracted from a Gas Phase
9.2 The STR 0perated as a Batch or as a Fed-Batch Reactor
9.2.1 The Batch Reactor
9.2.2 The Fed-Batch Reactor
9.3 Non-steady-State 0peration of the CSTR
9.3.1 Relations Between Cultivation Variables During Transients
9.3.2 The Stare Vector[s,x,p]in a Transient CSTR Experiment
9.3.3 Pulse Addition of Substrate to a CSTR.Stability of the Steadv State
9.3.4 Several Microorganisms Coinhabit the CSTR
9.3.5 The CSTR Used to Study Fast Transients
9.4 The Plug Flow Reactor
9.4.1 A CSTR Followed by a PFR
9.4.2 Loop Reactors
Problems
References
10 Gas-Liquid Mass Transfer
10.1 The Physical Processes Involved in Gas to Liquid Mass Transfer
10.1.1 Description of Mass Transfer Using k1a
10.1.2 Models for k1
10.1.3 Models for the Interfacial Area,and for Bubble Size
10.2 Empirical Correlations for k1a
10.3 Experimental Techniques for Measurement of O2 Transfer
10.3.1 The Direct Method
10.3.2 The Dynamic Method
10.3.3 The Sulfite Method
10.3.4 The Hydrogen Peroxide Method
10.3.5 k1 ObtaiBed by Comparison with the MassTransfer Coefficient of Other Gases
Problems
References
11 Scale-Up of Bioprocesses
11.1 Mixing in Bioreactors
11.1.1 Characterization of Mixing Efficiency
11.1.2 Experimental Determination of Mixing Time
11.1.3 Mixing Systems and Their Power Consumption
11.1.4 Power Input and Mixing for High Viscosity Media
11.1.5 Rotating Jet Heads:An Altemative to Traditional Mixers
11.2 Scale-Up Issues for Large Industrial Bioreactors
11.2.1 Modeling the Large Reactor Through Studies in Small Scale
11.2.2 Scale-Up in Practice:The Desirable and the Compromises
Problems
References
Index
List of Examples
List of Tables
List of Notes]]>
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