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分子生物学精要(影印版)
  • 书号:9787030103123
    作者:(美)马拉辛斯基(Malacinski,G.M.),(美)弗赖费尔德(Freifelder,D.)
  • 外文书名:
  • 装帧:平装
    开本:大16
  • 页数:532
    字数:788000
    语种:zh-Hans
  • 出版社:科学出版社
    出版时间:2002-04-01
  • 所属分类:Q7 分子生物学 Q78 基因工程(遗传工程) 0710 生物学
  • 定价: ¥118.00元
    售价: ¥93.22元
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本书深入浅出地阐明了蛋白质和核酸等生物大分子的结构、功能、协调和实验操作。
  书中简明扼要、图文并茂地提炼了日新月异的分子生物学进展,着重培养学生分析数据、解决问题和实验设计能力。书中还精选分子生物学家传略,附章前提示和章末小结、各类习题和解答、名词概念解释、参考文献及主题索引。
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目录

  • brief contents
    Prologue xv
    Preface xvii
    CHAPTER 1 Welcome to Molecular B iology! 3
    PART 1 The Structure of Proteins, Nucleic Acids, and Macromolecular Complexes 21
    CHAPTER 2 Macromolecules 23
    CHAPTER 3 Nucleic Acids 39
    CHAPTER 4 The Physical Structure of Protein Molecules 67
    CHAPTER 5 Macrom olecular Interactions and the Structure of Complex Aggregates 87
    CHAPTER 6 The Genetic Material 109
    CHAPTER 7 DNA Replication 131
    CHAPTER 8 Transcription 163
    CHAPTER 9 Translation 187
    CHAPTER 10 Mutations, Mutagenesis, and DNA Repair 213
    CHAPTER 11 Regulation of Gene Activity in Prokaryotes 251
    CHAPTER 12 Bacteriophage 281
    CHAPTER 13 Regulation of Gene Activity in Eukaryotes 311
    CHAPTER 14 Plasmids and Transposons 351
    CHAPTER 15 Recom binant DNA and Genetic Engineering: Molecular Tailoring of Genes 381
    CHAPTER 16 Molecular Biology Is Expanding Its Reach 409
    APPENDIX Chemical Principles Important for Understanding Molecular Biology 442
    List of Essential Concepts of Molecular Biology 471
    Glossary 475
    References for Further Reading 491
    Answers to Questions and Problems 497
    Index 515
    contents
    Prologue xv
    Preface xvii
    CHAPTER 1 Welcome to Molecular Biology! GEORGE M.MALACINSKI 3
    Goals of Molecular Biology 3
    The Early Years4
    Model Biological Systems 5
    Methodology of Molecular Biology 9
    Rapid Progress in Molecular Biology 12
    Putting the Details of Molecular Biology in Perspective 14
    Concepts of Molecular Biology 16
    Progression Diagram 17
    Rewards from Studying Molecular Biology 17
    PART I The Structure of Proteins, Nucleic Acids, and Macromolecular Complexes 21
    CHAPTER 2 Macromolecules GEORGE M.MALACINSKI 23
    Chemical Structures of the Major Classes of Macromolecules24
    Noncovalent Interactions that Determine the Three-dimensional Strnrtnrac of Prntainc and Nnrleic Acids 28
    Macromolecule Isolation and Characterization 31
    CHAPTER 3 Nucleic Acids ROBERT CEDERGREN 39
    Physical and Chemical Structure of DNA 40
    Alternate DNA Structures 43
    Circular and Superhelical DNA 45
    Renaturation 50
    Hybridization 53
    The Structure of RNA 55
    Hydrolysis of Nucleic Acids 56
    Sequencing Nucleic Acids 58
    Synthesis of DNA 60
    A Future Practical Application? 62
    CHAPTER 4 The Physical Scture of Protein Molecules ALEXANDRA CYNTHIA NEwToe 61
    Basic Features of Protein Molecules 68
    The Folding of a Polypeptide Chain 68
    The a Helix and Structures 70
    Protein Structure 73
    Proteins with Subunits 76
    Enzymes 79
    A Future Application 82
    CHAPTER 5 Macromolecular Interactions and the Structure of Complex Aggregates GEORGE M. MALACINSKI 87
    A Complex DNA Structure: The E. Coli Chromosome 88
    Chromosomes and Chromatin 91
    Interaction of DNA and Proteins that Recognize a Specific Base Sequence 96
    Biological Membranes 99
    Cytoskeletal Elements 102
    AFuture Practical Application? 103
    PART II Function of Macromolecules 107
    CHAPTER 6 The Genetic Material ROBERT L. SINSHEIMER 109
    Early Observations on the Mechanism of Heredity 110
    Identification of DNA as the Genetic Material 112
    Identification of RNA as the Genetic Material of Certain Viruses 120
    Properties of the Genetic Material 121
    RNA as the Genetir Material 127
    CHAPTER 7 DNA Replication_KENNETH MARIANS 131
    Semiconservative Replication of Double-Stranded DNA 132
    Untwisting of Highly Coiled DNA Is Required for DNA Replication 133
    Initiation of DNA Replication 136
    Unwinding of DNA for Replication 139
    Elongation of Newly Synthesized Strands 140
    DNA Polymerase III Consists of Multiple Subunits 143
    Antiparallel DNA Strands and Discontinuous Replication 145
    The Complete DNA Replication System 152
    Replication of Eukaryotic Chromosomes 156
    A Future Practical Avvlication? 158
    CHAPTE 8 Transcription Jonn RICHARDSON 163
    Enzymatic Synthesis of RNA 164
    Transcription Signals 166
    Classes of RNA Molecules 171
    Transcription in Eukaryotes 174
    Means of Studying Intracellular RNA 180
    A Future Practical Avolication? 182
    CHAPTER 9 Translation JOSEPH LAN 187
    Outline of Translation 188
    The Genetic Code 189
    The Wobble Hypothesis 194
    Polycistronic mRNA196
    Overlapping Genes 196
    Polypeptide Synthesis 198
    Stages of Polypeptide Synthesis in Prokaryotes 200
    Complex Translation Units 204
    Antibiotics 208
    A Future Practical Avvlication? 208
    CHAPTER 10 Mutations, Mutagenesis, and DNA Repair PHILIP C. HANAWALT 213
    Types of Mutations 214
    Biochemical Basis of Mutants 216
    Mutagenesis 218
    Spontaneous Mutations and Their Repair 220
    Induced Mutations 223
    Reversion 229
    Reversion as a Means of Detecting Mutagens and Carcinogens 234
    DNA Repair Mechanisms 236
    Repair by Direct Reversal 236
    Excision-Repair 237
    Recombinational Repair 238
    The SOS Response 240
    Future Practical Applications? 243
    PART III Coordination of Macromolecular Function in Cells 249
    CHAPTER 11 Regulation of Gene Activity in Prokaryotes SANKAR L.ADHYA 251
    Principles of Regulation 252
    Transcriptional Regulation 253
    Post-Transcriptional Control 272
    Feedback Inhibition and Allosteric Control 274
    A Future Practical Application? 276
    CHAPTER 12 Bacteriophage DAVID PARMA AND LARRY GOLD 281
    Stages in the Lytic Life Cycle of a Typical Phage 283
    Specific Phage 286
    Transducing Phage304
    A Future Practical Application? 307
    CHAPTER 13 Regulation of Gene Activity in Eukaryotes HANS-PETER MULLER AND WALTER SCHAFFNER 311
    Important Differences in the Genetic Organization of Prokaryotes and Eukaryotes 312
    The Regulation of Transcription Initiation 313
    The Regulation of RNA Processing 324
    Regulation of Nucleocytoplasmic mRNA Transport 330
    Regulation of mRNA Stability 331
    Regulation of Translation 334
    Regulation of Protein Activity 335
    Gene Rearrangement:Joining of Coding Sequences in the Immune System 340
    A Future Practical Application? 344
    PART IV Experimental Manipulation of Macomolecules 349
    CHAPTER 14 Plasmids and Transposons BARRY POLISKY 351
    Plasmid-Borne Genes 353
    Plasmid Transfer 354
    Plasmid DNA Replication 361
    Transposable Elements 364
    Transposable Elements in Eukaryotes 374
    A Future Practical Application? 375
    CHAPTER 15 Recombinant DNA and Genetic Engineering: Molecular Tailoring of Genes DOROTHY M.SKINNER AND CHRISTIE A.HOLLAND 381
    Plasmids Act as Nature's Interlopers 382
    Restriction Enzymes Function as Nature's Pinking Shears 382
    Genetic Interlopers:Vectors Function as Vehicles for Transferring Genes 392
    Detection of Recombinant DNA Molecules 399
    Applications of Genetic Engineering 403
    CHAPTER 16 Molecular Biology IS Expanding ITS Reach PAMELA L.HANRATTY 409
    Uses of Recombinant DNA Technology in Research 410
    Uses of Recombinant DNA Technology in Medicine 417
    Uses of Recombinant DNA Technology in Agriculture 427
    Other Commercial and Industrial Applications 430
    Molecular Biology: On the Front Line of the Battle Against AIDS 431
    Social and Ethical Issues 433
    APPENDIX Chemical Principles Important for Undestanding Molecular Biology JUDITH A.JAEHNING 443
    Structure of the Atom 444
    Chemical Bonds 444
    The Ionization of Water-The pH Scale 450
    Organic Chemistry 453
    Concluding Note 469
    List of Essential Concepts 471
    Glossary 475
    References for Further Reading 491
    Answers to Questions and Problems 497
    Index 515
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