目录 第一部分 植物细胞遗传学的结构、变异和图谱 1. 植物染色体的删除、插入和重排 3 2. 基因组的结构和染色体功能 37 3. 植物B型染色体:是什么使它们不同? 59 4. 植物的细胞遗传学图谱 79 5. DNA和染色质纤维为基础的植物细胞遗传学 121 第二部分 植物细胞遗传学的功能、组织和动力学 6. 植物着丝粒 133 7. 植物端粒 143 8. 植物减数分裂期染色体的遗传和细胞学行为 193 9. 表观遗传组蛋白标记在植物染色体上的分布和功能解释 231 第三部分 植物细胞遗传学的方法、信息和指导教学 10. 染色体的显微切割和显微分离DNA技术的应用 257 11. 玉米抗体操作手册:免疫定位和染色质免疫共沉淀实验 271 12. 延伸DNA纤维的荧光原位杂交技术 287 13. 荧光原位杂交和荧光原位PCR技术 295 14. 植物细胞遗传学的基因组数据库 311 15. 植物分子细胞遗传学的实验室实践练习 323 索引 335 (李建勇 译) Contents Part I Structure,Variation,and Mapping in Plant Cytogenetics 1 Plant Chromosomal Deletions,Insertions,and Rearrangemens 3 Donald L.Auger and William F.Sheridan 2 Genome Structure and Chromosome Function 37 Khwaja G.Hossain,Scott A.Jackson,and Shahryar F.Kianian 3 Plant B Chromosomes: What Makes Them Different? 59 Andreas Houben and Mariana Carchilan 4 Cytogenetic Mapping in Plants 79 Debbie M.Figueroa,Ina E.Amarillo,and Hank W.Bass 5 DNA and Chromatin Fiber-Based Plant Cytogenetics 121 Jason G.Walling and Jiming Jiang Part II Function,Organization,and Dynamics in Plant Cytogenetics 6 Plant Centromeres 133 James A.Birchler,Zhi Gao,and Fangpu Han 7 Plant Telomeres 143 Jonathan C.Lamb,Eugene V.Shakirov,and Dorothy E.Shippen 8 Genetics and Cytology of Meiotic Chromosome Behavior in Plants 193 Shaun P.Murphy and Hank W.Bass 9 Chromosomal Distribution and Functional Interpretation of Epigenetic Histone Marks in Plants 231 Jorg Fuchs and Ingo Schubert Part III Methods,lnformatics,and Instruction in Plant Cytogenetics 10 Chromosome Microdissection and Utilization of Microisolated DNA 257 Andreas Houben 11 Maize Antibody Procedures:Immunolocalization and Chromatin Immunoprecipitatio 271 X.Li,C.N.Topp,and R.K.Dawe 12 Methods of Fluorescence In Situ Hybridization on Extended DNA Fibers (Fiber-FISH) 287 Jason G.Walling,Robert M.Stupar,and Jiming Jiang 13 Fluorescence In Situ Hybridization and In Situ PCR 295 James A.Birchler and Tatiana V.Danilova 14 Plant Cytogeneticsin Genome Databases 311 Lisa C.Harper,Taner Z.Sen,and Carolyn J.Lawrence 15 Practical Laboratory Exercises for Plant Molecular Cytogenetics 323 Scott A.Jackson,Shahryar F.Kianian,Khwaja G.Hossain,and Jason G.Walling Index 335